enzyme a Search Results


otud5  (Proteintech)
93
Proteintech otud5
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Otud5, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/OTUD5+Antibody/bio_rxiv__2022__01__11__475808-154-50-52
Average 93 stars, based on 1 article reviews
otud5 - by Bioz Stars, 2026-09
93/100 stars
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anti angiotensin converting enzyme 2 ace 2  (ProSci Incorporated)
91
ProSci Incorporated anti angiotensin converting enzyme 2 ace 2
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Anti Angiotensin Converting Enzyme 2 Ace 2, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/ADAM17+Antibody/pmc08110335-357-22-26
Average 91 stars, based on 1 article reviews
anti angiotensin converting enzyme 2 ace 2 - by Bioz Stars, 2026-09
91/100 stars
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coash  (Chem Impex International)
95
Chem Impex International coash
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Coash, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/Coenzyme+A+hydrate/pm28414710-469-0-3
Average 95 stars, based on 1 article reviews
coash - by Bioz Stars, 2026-09
95/100 stars
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yeast lytic enzyme a. g. scientific  (AG Scientific)
90
AG Scientific yeast lytic enzyme a. g. scientific
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Yeast Lytic Enzyme A. G. Scientific, supplied by AG Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/yeast+lytic+enzyme+a++g++scientific/pm37285383-236-8-11
Average 90 stars, based on 1 article reviews
yeast lytic enzyme a. g. scientific - by Bioz Stars, 2026-09
90/100 stars
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enzyme a buzyme 7703  (Buckman Laboratories)
90
Buckman Laboratories enzyme a buzyme 7703
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Enzyme A Buzyme 7703, supplied by Buckman Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/enzyme+a+buzyme+7703/10__1590_slash_s0104___66322011000300003-26-0-4
Average 90 stars, based on 1 article reviews
enzyme a buzyme 7703 - by Bioz Stars, 2026-09
90/100 stars
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sized amylolytic enzyme a-amylase  (Verlag GmbH)
90
Verlag GmbH sized amylolytic enzyme a-amylase
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Sized Amylolytic Enzyme A Amylase, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/sized+amylolytic+enzyme+a+amylase/pm15990960-5-14-8
Average 90 stars, based on 1 article reviews
sized amylolytic enzyme a-amylase - by Bioz Stars, 2026-09
90/100 stars
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co-enzyme a nanolight 309  (NanoLight Inc)
90
NanoLight Inc co-enzyme a nanolight 309
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Co Enzyme A Nanolight 309, supplied by NanoLight Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/co+enzyme+a+nanolight+309/pmc11650635-53-0-3
Average 90 stars, based on 1 article reviews
co-enzyme a nanolight 309 - by Bioz Stars, 2026-09
90/100 stars
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enzyme a-galactosidase a (a-gal)  (Marburg GmbH)
90
Marburg GmbH enzyme a-galactosidase a (a-gal)
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Enzyme A Galactosidase A (A Gal), supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/enzyme+a+galactosidase+a++a+gal+/10__1093_slash_eurheartj_slash_26__suppl_1__205-11882-28-7
Average 90 stars, based on 1 article reviews
enzyme a-galactosidase a (a-gal) - by Bioz Stars, 2026-09
90/100 stars
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enzyme a  (Medox Inc)
90
Medox Inc enzyme a
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Enzyme A, supplied by Medox Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/enzyme+a/10__1016_slash_b978___012373738___0__50014___3-70-32-47
Average 90 stars, based on 1 article reviews
enzyme a - by Bioz Stars, 2026-09
90/100 stars
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a-aylmethyl co-enzyme a racemase amacr antibody  (Zeta Corp)
90
Zeta Corp a-aylmethyl co-enzyme a racemase amacr antibody
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
A Aylmethyl Co Enzyme A Racemase Amacr Antibody, supplied by Zeta Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/a+aylmethyl+co+enzyme+a+racemase+amacr+antibody/pmc02739832-94-16-21
Average 90 stars, based on 1 article reviews
a-aylmethyl co-enzyme a racemase amacr antibody - by Bioz Stars, 2026-09
90/100 stars
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mouse cgas enzyme a recombinant dna  (GenScript corporation)
90
GenScript corporation mouse cgas enzyme a recombinant dna
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
Mouse Cgas Enzyme A Recombinant Dna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/mouse+cgas+enzyme+a+recombinant+dna/us11203610-1016-4-17
Average 90 stars, based on 1 article reviews
mouse cgas enzyme a recombinant dna - by Bioz Stars, 2026-09
90/100 stars
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the mechanism 0/ enzyme a ction  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare the mechanism 0/ enzyme a ction
A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between <t>OTUD5</t> and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.
The Mechanism 0/ Enzyme A Ction, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/enzyme+a/the+mechanism+0++enzyme+a+ction/10__1146_slash_annurev__bi__30__070161__002023-1057-8-17
Average 90 stars, based on 1 article reviews
the mechanism 0/ enzyme a ction - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between OTUD5 and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.

Journal: bioRxiv

Article Title: Hypoxia Dynamically Regulates DBC1 Ubiquitination and Stability by SIAH2 and OTUD5 in Breast Cancer Progression

doi: 10.1101/2022.01.11.475808

Figure Lengend Snippet: A, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in MDA-MB-231 cells under hypoxia for 12 h and 24 h and then recovered to normoxia for 6 h. B, Co-IP analysis showing the endogenous interaction between OTUD5 and DBC1 in MDA-MB-231 cells. C, GST pulldown analysis showing the interaction between His-tagged DBC1 and endogenous OTUD5 immunoprecipitated from MDA-MB-231 cells. D, Co-IP analysis showing the ubiquitination level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. E, Western blotting analysis showing the protein level of endogenous DBC1 in HeLa cells transfected with wild-type Falg-OTUD5 or Flag-OTUD5 CS under hypoxia. F-G, Western blotting analysis showing the half-life of endogenous DBC1 in HeLa cells transfected with wild-type Flag-OTUD5 or Flag-OTUD5 CS and then treated with CHX (10 μM) under hypoxia. F, Quantification of DBC1 protein levels as shown in ( G ). H, Mapping the domain of DBC1 interacting with Flag-OTUD5 in HEK293T cells cotransfected with full-length or truncated forms of Myc-DBC1 and Flag--OTUD5. I, Co-IP analysis showing the endogenous interaction between DBC1 and SIAH2 or OTUD5 in MDA-MB-231 cells under hypoxia for 24 h. J, Schematic model presenting the competitive interaction of SIAH2 and OTUD5 with DBC1 under hypoxia.

Article Snippet: The following antibodies were used: Flag-M2 (1:2000, Sigma), Myc (1:1000, Santa Cruz Biotech.), HA (1:1000, Santa Cruz Biotech.), GFP (1:1000, Santa Cruz Biotech.), His (1:1000, Santa Cruz Biotech.), GST (1:1000, Santa Cruz Biotech.), anti-ACTIN (1:10000, Sigma), anti-mono- and polyubiquitinated conjugate monoclonal antibodies (FK2) (1:1000, Enzo Life Sciences), SIAH2 (1:500 Proteintech), OTUD5 (1:1000 Proteintech & CST), DBC1 (1:1000 Proteintech & CST & Abcam), SIRT1 (1:1000 Abcam), p53 (1:1000 Abcam), acetylated-K382-p53 (1:1000 Abcam), HIF1α (1:1000 Proteintech), BAX (1:1000 Proteintech), p21 (1:1000 Proteintech), and GSK3β (1:1000 Proteintech).

Techniques: Co-Immunoprecipitation Assay, Ubiquitin Proteomics, Immunoprecipitation, Transfection, Western Blot

A, Western blotting analysis of the expression levels of the indicated DUBs in HeLa cells transfected with the indicated Flag-tagged DUBs. B-C, Co-IP analysis of the interaction between DBC1 and OTUD5 in HEK293T cells cotransfected with Myc-DBC1 and Flag-OTUD5. D, Co-IP analysis of the interaction between DBC1 and SIAH2 and the interaction between DBC1 and OTUD5 in HEK293T cells cotransfected with Myc-DBC1, Flag-OTUD5 and Flag-SIAH2 RM at the indicated dosages.

Journal: bioRxiv

Article Title: Hypoxia Dynamically Regulates DBC1 Ubiquitination and Stability by SIAH2 and OTUD5 in Breast Cancer Progression

doi: 10.1101/2022.01.11.475808

Figure Lengend Snippet: A, Western blotting analysis of the expression levels of the indicated DUBs in HeLa cells transfected with the indicated Flag-tagged DUBs. B-C, Co-IP analysis of the interaction between DBC1 and OTUD5 in HEK293T cells cotransfected with Myc-DBC1 and Flag-OTUD5. D, Co-IP analysis of the interaction between DBC1 and SIAH2 and the interaction between DBC1 and OTUD5 in HEK293T cells cotransfected with Myc-DBC1, Flag-OTUD5 and Flag-SIAH2 RM at the indicated dosages.

Article Snippet: The following antibodies were used: Flag-M2 (1:2000, Sigma), Myc (1:1000, Santa Cruz Biotech.), HA (1:1000, Santa Cruz Biotech.), GFP (1:1000, Santa Cruz Biotech.), His (1:1000, Santa Cruz Biotech.), GST (1:1000, Santa Cruz Biotech.), anti-ACTIN (1:10000, Sigma), anti-mono- and polyubiquitinated conjugate monoclonal antibodies (FK2) (1:1000, Enzo Life Sciences), SIAH2 (1:500 Proteintech), OTUD5 (1:1000 Proteintech & CST), DBC1 (1:1000 Proteintech & CST & Abcam), SIRT1 (1:1000 Abcam), p53 (1:1000 Abcam), acetylated-K382-p53 (1:1000 Abcam), HIF1α (1:1000 Proteintech), BAX (1:1000 Proteintech), p21 (1:1000 Proteintech), and GSK3β (1:1000 Proteintech).

Techniques: Western Blot, Expressing, Transfection, Co-Immunoprecipitation Assay